Journal: bioRxiv

Article Title: Adipose Tissue Inflammation, Oxidative Stress, and Altered Adipogenesis Are Drivers of Dyslipidemia – A Multi-Omics Overview of Dyslipidemia in Obesity

doi: 10.1101/2025.10.15.682716

Figure Lengend Snippet: Correlation plots of leptin with HDL-C and ApoAI with lines of best fit and confidence interval. Spearman’s rank correlation (r), and its Benjamini-Hochberg adjusted p-value ( p ), only significant correlations depicted.

Article Snippet: Plasma adiponectin and leptin concentrations were measured using human Adiponectin/Acrp30 DuoSet ® ELISA (Y1065-05; R&D Systems, Minneapolis, USA) and human Leptin DuoSet ® ELISA (DY398-05; R&D Systems, Minneapolis, USA).

Techniques:

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: Comparison of circulating leptin ( A ), FLI ( B ), sOB-R ( C ) and ghrelin ( D ) pre-surgery and post-surgery (week 1 until 12 months post-surgery). Statistically significant differences were observed in groups post-surgery, * p < 0.01; ** p < 0.001; *** p < 0.005; **** p < 0.0001.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: ( A ) Correlation matrix between circulating leptin, ghrelin, FLI, and anthropometric/metabolic variable changes following metabolic and bariatric surgery. Values are repeated-measures correlations (also called intra-individual correlations). Only significant results are shown based on Bonferroni-adjusted p -value. ( B ) Graph of correlations of sOB-R and FLI with leptin.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: The Journal of veterinary medical science

Article Title: Sandwich enzyme-linked immunosorbent assay of canine leptin.

doi: 10.1292/jvms.62.207

Figure Lengend Snippet: Fig. 1. SDS-PAGE and Western blot analyses of canine leptin. Recombinant canine (D), rat (R), mouse (M) and human (H) leptins (200 ng/lane) were electrophoresed through SDS-polyacrylamide gel (5–20%), and detected by either Coomassie brilliant blue R250 (top panel), anti- canine leptin antibody (second panel), anti-human leptin antibody (third panel), or anti-mouse leptin antibody (bottom panel).

Article Snippet: SDS-polyacrylamide gel (5–20%) electrophoresis (SDSPAGE) analysis revealed that the recombinant canine leptin migrated similarly to recombinant leptins of rat (R&D Systems, Minneapolis, MN, U.S.A.), mouse (PeproTechEC Ltd., London, England) and human (PeproTechEC), at the position of about 16 kDa (Fig. 1, top panel).

Techniques: SDS Page, Western Blot, Recombinant

Journal: The Journal of veterinary medical science

Article Title: Sandwich enzyme-linked immunosorbent assay of canine leptin.

doi: 10.1292/jvms.62.207

Figure Lengend Snippet: Fig. 2. Standard curve of sandwich ELISA using recombinant canine leptin. Recombinant canine leptin (0.5–32 ng/ml) was measured by a sandwich ELISA using purified anti-canine leptin antibody.

Article Snippet: SDS-polyacrylamide gel (5–20%) electrophoresis (SDSPAGE) analysis revealed that the recombinant canine leptin migrated similarly to recombinant leptins of rat (R&D Systems, Minneapolis, MN, U.S.A.), mouse (PeproTechEC Ltd., London, England) and human (PeproTechEC), at the position of about 16 kDa (Fig. 1, top panel).

Techniques: Sandwich ELISA, Recombinant, Purification

Journal: Pediatric Reports

Article Title: Resistin in Urine and Breast Milk: Relation to Type of Feeding and Anthropometry at 1-Month

doi: 10.3390/pediatric14010013

Figure Lengend Snippet: Correlation between resistin concentration in the breast milk and ( a ) body weight; ( b ) age; ( c ) body mass index (BMI); and ( d ) leptin concentration in mothers’ breast milk at 1 month post-partum.

Article Snippet: We analyzed urinary and breast milk resistin levels (Human Resistin ELISA kit; Arigo biolaboratories, Hsinchu City, Taiwan) and breastmilk leptin (Human Leptin Quantikine ELISA Kit; R&D Systems, Minneapolis, MI, USA) in infants using an enzyme-linked immunosorbent assay kit.

Techniques: Concentration Assay

Journal: Biomolecules

Article Title: Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation

doi: 10.3390/biom11020325

Figure Lengend Snippet: Levels of plasma adipocytokines and chemokines.

Article Snippet: Total and HMW adiponectin, leptin, and resistin in plasma were measured using the following enzyme-linked immunosorbent assays (ELISA): Human Total Adiponectin/Acrp30 Quantikine ELISA, Human HMW Adiponectin/Acrp30 Quantikine ELISA, Human Leptin Quantikine ELISA, and Human Resistin Quantikine ELISA (R&D Systems, Bio-Techne, Minneapolis, MN, USA).

Techniques: Clinical Proteomics