Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Adipokine Profile in Patients with Type 2 Diabetes Depends on Degree of Obesity

doi: 10.12659/MSM.904318

Figure Lengend Snippet: Leptin/adiponectin ratio in control group and type 2 diabetic patients divided according to BMI value. K – control group, group I – type 2 diabetic patients with normal body weight, group II – type 2 diabetic patients with overweight, group III – type 2 diabetic patients with obesity and group IV – type 2 diabetic patients with severe obesity. * p<0.05 vs. group K; ** p<0.01 vs. group K; # p<0.05 vs. group I.

Article Snippet: The plasma concentrations of the studied adipokines were determined by immunoenzymatic methods using Human Leptin Quantikine ELISA Kit (R&D Systems, USA), Human Total Adiponectin/Acrp30 Quantikine ELISA (R&D Systems, USA), and Human Resistin Quantikine ELISA Kit (R&D Systems, USA), as well as Human Nicotinamide phosphoribosyltransferase, NAmPRTase ELISA Kit (Wuhan EIAab Science, China) for leptin, adiponectin, resistin, and visfatin, respectively.

Techniques: Control

Journal: European Journal of Inflammation

Article Title: Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

doi: 10.1177/2058739218774975

Figure Lengend Snippet: Figure 4. LPS influence on TNF-α secretion. SAT explants derived from each patient were incubated in triplicate with or without 10 µg/mL of LPS for (a) 2 h and (b) 20 h. Secreted quantities of leptin in media were determined by ELISA. Results were depicted as relative quantities (RQs) compared to the control (without LPS; C). ****P < 0.0001 versus control. Error bars, SEM (N = 8).

Article Snippet: Human leptin DuoSet enzymelinked immunosorbent assay (ELISA) kit, substrate solution were purchased from R&D systems (USA), and ELISA plates were purchased from Greiner Bio-One (Germany).

Techniques: Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: European Journal of Inflammation

Article Title: Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

doi: 10.1177/2058739218774975

Figure Lengend Snippet: Figure 2. Role of BMI in leptin response. SAT explants derived from each patient were incubated in triplicate for 20 h with or without 10 µg/mL of LPS. (a) The correlation between BMI and leptin response to LPS was determined employing Pearson correlation test. Pearson correlation coefficient was 0.8 and *P < 0.05. (b) Explants derived from low BMI patients (BMI ⩽ 25) responded to LPS treatment, while explants derived from high BMI patients (BMI > 25) did not respond. Secreted quantities of leptin in media were determined by ELISA. Results were depicted as relative quantities (RQs) compared to the control (without LPS; C). ****P < 0.0001 versus control. #P < 0.0001 versus BMI ⩽ 25. Error bars, SEM (N = 9).

Article Snippet: Human leptin DuoSet enzymelinked immunosorbent assay (ELISA) kit, substrate solution were purchased from R&D systems (USA), and ELISA plates were purchased from Greiner Bio-One (Germany).

Techniques: Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: European Journal of Inflammation

Article Title: Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

doi: 10.1177/2058739218774975

Figure Lengend Snippet: Figure 3. Role of (a) age and (b) gender in leptin response. SAT explants derived from each patient were incubated in triplicate for 20 h with or without 10 µg/mL of LPS. (a) Role of age, the correlation between age and leptin response to LPS was determined employing Pearson correlation test. Pearson correlation coefficient was 0.3 (P = 0.46). (b) Role of gender: patients were divided into two groups according to their gender. Secreted quantities of leptin in media were determined by ELISA. Results were depicted as relative quantities (RQs) compared to the control (without LPS; C, N = 9).

Article Snippet: Human leptin DuoSet enzymelinked immunosorbent assay (ELISA) kit, substrate solution were purchased from R&D systems (USA), and ELISA plates were purchased from Greiner Bio-One (Germany).

Techniques: Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: European Journal of Inflammation

Article Title: Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

doi: 10.1177/2058739218774975

Figure Lengend Snippet: Figure 1. LPS influence on leptin secretion. SAT explants derived from each patient were incubated in triplicate with or without 10 µg/mL of LPS for (a) 2 h (N = 10) and (b) 20 h (N = 9). Secreted quantities of leptin in media were determined by ELISA. Results were depicted as relative quantities (RQs) compared to the control (without LPS; C). **P < 0.01 versus control. Error bars, SEM.

Article Snippet: Human leptin DuoSet enzymelinked immunosorbent assay (ELISA) kit, substrate solution were purchased from R&D systems (USA), and ELISA plates were purchased from Greiner Bio-One (Germany).

Techniques: Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: European Journal of Inflammation

Article Title: Lipopolysaccharide influence on leptin hormone and tumor necrosis factor-alpha release from human adipose tissue

doi: 10.1177/2058739218774975

Figure Lengend Snippet: Figure 5. Role of (a) BMI and (b) age in TNF-α response. SAT explants derived from each patient were incubated in triplicate for 20 h with or without 10 µg/mL of LPS. Correlation between BMI and age on one hand and TNF-α response to LPS on the other hand was determined employing Pearson correlation test. Pearson correlation coefficients were −0.4 (P = 0.32) and 0.2 (P = 0.62) for both BMI and age, respectively. Secreted quantities of leptin in media were determined by ELISA. Results were depicted as relative quantities (RQs) compared to the control (without LPS; C, N = 8).

Article Snippet: Human leptin DuoSet enzymelinked immunosorbent assay (ELISA) kit, substrate solution were purchased from R&D systems (USA), and ELISA plates were purchased from Greiner Bio-One (Germany).

Techniques: Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of Pediatric Gastroenterology & Nutrition

Article Title: Hyperadiponectinemia During Infliximab Induction Therapy in Pediatric Crohn Disease

doi: 10.1097/mpg.0000000000001876

Figure Lengend Snippet: FIGURE 1. Serum adipokine levels according to sex in healthy controls and in CD patients at baseline (week 0) and under infliximab therapy (weeks 2 and 14) displaying each patient individually and the median lines (n ¼ 18). (A) Resistin levels were higher in CD at baseline, decreased under therapy and showed no sex differences. (B) Leptin was significantly higher in girls at all time points and increased under IFX. (C) Adiponectin tended to be higher in girls, peaked 2 weeks after first IFX infusion and thereafter decreased. Differences were analyzed with Wilcoxon matched- pairs signed rank test. P < 0.05, P < 0.01, P < 0.001, P < 0.0001. CD ¼ Crohn disease, HC ¼ healthy controls, IFX ¼ infliximab.

Article Snippet: Resistin, leptin (Human Leptin and Resistin Quantikine ELISA Kits, R&D Systems, Minneapolis, MN) and total adiponectin (Total Adiponectin ELISA Kit, ALPCO Diagnostics, Salem, NH) serum levels were assessed with commercially available enzyme-linked immunosorbent assays (ELISA) according to the manufacturer’s instructions.

Techniques:

Journal: BMC women's health

Article Title: Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators reveal a pro-inflammatory state in overweight women.

doi: 10.1186/s12905-025-03588-4

Figure Lengend Snippet: Fig. 1 Levels of circulating markers of inflammation are altered in overweight women compared with lean women. Plasma samples of overweight and lean subjects were analyzed for (A) LPB; *P = 0.017, (B) CRP; *P = 0.023, (C) IL-6; *P = 0.016, (D) leptin; **P = 0.004, and (E) adiponectin; **P = 0.006, as described in Materials and Methods, and leptin/adiponectin ratios; ***P = 0.0007 (F) were calculated. Data are shown as median with interquartile range

Article Snippet: Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection ((LLoD) 15.6pg/ml), C-reactive protein or CRP by ELISA (DY1707, R&D Systems, LLoD 15.6pg/ml); leptin by ELISA (DY398-05, R&D Systems, LLoD 31.2pg/ml); LPS binding protein or LBP by ELISA (DY870-05, R&D Systems, LLoD 0.8ng/ml); and IL-6 using the V-plex MSD platform (K151QXD-2, Meso Scale Discovery, LLoD 0.06pg/ml).

Techniques: Clinical Proteomics

Journal: BMC women's health

Article Title: Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators reveal a pro-inflammatory state in overweight women.

doi: 10.1186/s12905-025-03588-4

Figure Lengend Snippet: Fig. 2 Correlation of plasma inflammatory indices and BMI. Plasma LBP (A), CRP (B), IL-6 (C), leptin (D), and adiponectin (E) levels significantly correlate with BMI as indicated by P values and Spearman correlation coefficient (r)

Article Snippet: Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection ((LLoD) 15.6pg/ml), C-reactive protein or CRP by ELISA (DY1707, R&D Systems, LLoD 15.6pg/ml); leptin by ELISA (DY398-05, R&D Systems, LLoD 31.2pg/ml); LPS binding protein or LBP by ELISA (DY870-05, R&D Systems, LLoD 0.8ng/ml); and IL-6 using the V-plex MSD platform (K151QXD-2, Meso Scale Discovery, LLoD 0.06pg/ml).

Techniques: Clinical Proteomics

Journal: Molecular human reproduction

Article Title: Modulation of placental vascular endothelial growth factor by leptin and hCG.

doi: 10.1093/molehr/gag053

Figure Lengend Snippet: Figure 1. Release of hCG (% of controls) at 4 h from cytotrophoblastic cells incubated for 4 h with a range of concentrations of recombinant human leptin. Statistics were performed by analysis of variance and P values refer to differences compared with control (Leptin, 0). Error bars are SEM (n = 4 experiments in duplicate).

Article Snippet: Recombinant human leptin (rhLept) was purchased from R&D Systems (BuÈhlmann Laboratories, Switzerland). hCG (Profasi) was purchased from Ares±Serono (Switzerland).

Techniques: Incubation, Recombinant, Control

Journal: Molecular human reproduction

Article Title: Modulation of placental vascular endothelial growth factor by leptin and hCG.

doi: 10.1093/molehr/gag053

Figure Lengend Snippet: Figure 2. Release of vascular endothelial growth factor (VEGF) (% of controls) at 4 h from cytotrophoblastic cells incubated for 4 h with a range of concentrations of recombinant human leptin. Statistics were performed by analysis of variance and P values refer to differences compared with controls (Leptin, 0). Error bars are SEM (n = 4 experiments in duplicate).

Article Snippet: Recombinant human leptin (rhLept) was purchased from R&D Systems (BuÈhlmann Laboratories, Switzerland). hCG (Profasi) was purchased from Ares±Serono (Switzerland).

Techniques: Incubation, Recombinant

Journal: Molecular human reproduction

Article Title: Modulation of placental vascular endothelial growth factor by leptin and hCG.

doi: 10.1093/molehr/gag053

Figure Lengend Snippet: Figure 3. Release of leptin (% of controls) at 4 h from cytotrophoblastic cells incubated for 4 h with a range of concentrations of hCG (Profasi). Statistics were performed by analysis of variance and P values refer to differences compared with control (hCG, 0). Error bars are SEM (n = 4 experiments in duplicate).

Article Snippet: Recombinant human leptin (rhLept) was purchased from R&D Systems (BuÈhlmann Laboratories, Switzerland). hCG (Profasi) was purchased from Ares±Serono (Switzerland).

Techniques: Incubation, Control

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: Comparison of circulating leptin ( A ), FLI ( B ), sOB-R ( C ) and ghrelin ( D ) pre-surgery and post-surgery (week 1 until 12 months post-surgery). Statistically significant differences were observed in groups post-surgery, * p < 0.01; ** p < 0.001; *** p < 0.005; **** p < 0.0001.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: ( A ) Correlation matrix between circulating leptin, ghrelin, FLI, and anthropometric/metabolic variable changes following metabolic and bariatric surgery. Values are repeated-measures correlations (also called intra-individual correlations). Only significant results are shown based on Bonferroni-adjusted p -value. ( B ) Graph of correlations of sOB-R and FLI with leptin.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: